Purified lymphocytes were used for T-cell stimulation. T Lymphocyte Stimulation Assay. Analysis of TCR specific stimulation was performed using anti-CD3 and anti-CD28 cross-linking from 1-12 months post- transplant,. For TCR-specific stimulation, cells were incubated for 48 hours on plates previously coated with purified anti-CD3 (1-10 pg/ml) and anti-CD28 (10, are sarms legal in singapore. Following plaque formation (48-72 hours), 1 p,Ci of 3H-Thymidine was added to each well and plates incubated for a further 16-24 hours.Greub, Lausanne (Microbiologie clinique) ; Pr I, are sarms legal in singapore.
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